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DetailsCatalogue
Cell line:
PA-TU-8902
DSMZ no.:
ACC 179
Species:
human (Homo sapiens)
Cell type:
pancreas adenocarcinoma
Origin:
established in 1985 from the primary ductal pancreatic adenocarcinoma (grade II) from a 44-year-old woman; reported as inducing metastasis in nude mice and as secreting proteinases (e.g. urokinase, cathepsin B and D)
Reference(s):
14509, 18168
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial adherent cells growing in monolayers; image; image
Medium:
90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 25-40 hours
Harvest:
cell harvest of 1.0-1.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human flat-moded pseudotetraploid karyotype - 91(83-94)<4n>XXX-X, +5, -6, -6, +7, -10, -10, +12, -13, -13, -14, -19, +20, -22, -22, +5mar, del(4)(q26)x2, der(5)t(5;14)(p11;q11), der(5)t(5;?)(q11;?), der(7)t(7;?)(p14;?), der(9)t(9;11)(p12;q11), der(14)t(?5;14)(?p11;p11)x2, del(17)(p12), der(17)t(17;?)(p11/12;?), der(18)t(18;?)(p12;?), der(19)t(19;?)(q13.1;?), ?i(21q)x3, der(22)t(22;?)(q13;?) - carries 19p13.1 recurrent tumor breakpoint - corresponds to vague published description
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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