HEP-G2
ACC 180
Cell line:
HEP-G2
DSMZ no.:
ACC 180
Species:
human (Homo sapiens)
Cell type:
hepatoma/hepatoblastoma
Origin:
established from the tumor tissue of a 15-year-old boy in 1975 (originally described as a hepatocellular carcinoma but others suggest it as a hepatoblastoma); cells were described in the literature to not harbor a hepatitis B virus genome; cells reportedly produce a variety of proteins: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement (C3, C4), C3 activator, fibrinogen, alpha1-acid glycoprotein, alpha2-HS glycoprotein, ß-lipoprotein, retinol binding protein
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of about 10 x 106 cells/80 cm2,
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 52(47-54)<2n>XY, +2, +14, +17, +20, +2mar, t(1;21) (p22.2;p11-12), i(17q)/der(17)t(17;17)(p11;q11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list