Species:
human (Homo sapiens)
Cell type:
acute promyelocytic leukemia
Origin:
established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; a method of NB-4 transfection was patented in 2016; cells carry the t(15;17) PML-RARA fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3 days; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-45 hours
Harvest:
cell harvest of 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD3 -, CD4 +, CD11b -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD38 +, HLA-DR -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 3% polyploidy - 78(71-81)<3n>XX, -X, +2, +6, +7, +7, +11, +12, +13, +14, +17, -19, +20, +4mar, der(8)t(8;?)(q24;?), der(11)t(11;?)(?->::11p15->11q22.1::11q13->22.1:), der(12)t(12;?)(p11;?), 14p+, t(15;17)(q22;q11-12.1), der(19)t(10;19)(q21.1;p13.3)x2 - identity confirmed
Molec. Genetics:
expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
