Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 15-year-old girl with acute lymphoblastic leukemia (ALL at first relapse) in 1973; carries t(12;21) leading to ETV6-RUNX1 (TEL-AML1) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
cells may be difficult to culture! seed out at ca. 2 x 106 cells/ml with initially 20% FBS in a 24-well plate; cells can be maintained at high density (>2 x 106 cells/ml); minimum density at ca. 0.5 x 106 cells/ml; cells grow slowly; after thawing viability may decreases during the first days, but cells recover afterwards; do not change medium during this time
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximum density at ca. 3-5 x 106 cells/ml or higher
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 -, CD38 +, cyCD79a+, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype - 46(44-47)<2n>X, -X, +16, del(3)(p22), t(4;12;21;16)(q32;p13;q22;q24.3)-inv(12)(p13q22), t(5;12)(q31-q32;p12), der(16)t(16;21)(q24.3;q22) - sideline with inv(5)der(5)(p15q31),+18 - carries t(12;21) and del(12) producing respective ETV6-RUNX1 (TEL-AML1) fusion and deletion of residual ETC6 (TEL)
Molec. Genetics:
expression of fusion gene ETV6-RUNX1 (TEL-AML1) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
