Leibniz Institute DSMZ: Details

Cell line:

BL-70

DSMZ no.:

ACC 233

Species:

human (Homo sapiens)

Cell type:

Burkitt lymphoma

Origin:

established from the tumor tissue at relapse of a 16-year-old boy with Burkitt lymphoma; cells are EBV-negative

Reference(s):

Biosafety level:

1

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

single, round cells growing non-adherent in suspension; image; image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 µM mercaptoethanol)

Subculture:

optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at ca. 0.3 x 10⁶ cells/ml; start culture with 20% FBS after thawing

Incubation:

at 37 °C with 5% CO₂

Doubling time:

about 44 hours

Harvest:

saturation density of about 1.0 x 10⁶cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, +7, inv(1)(p21q21), del(2)(q33), t(8;14)(q24;q32), t(12;22)(q21.1;q13.2)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €