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DetailsCatalogue
Cell line:
SAOS-2
DSMZ no.:
ACC 243
Species:
human (Homo sapiens)
Cell type:
osteogenic sarcoma
Origin:
established from the primary osteogenic sarcoma of an 11-year-old Caucasian girl in 1973; cells secrete particulate matter containing bone inducing agents thought to comprise bone morphogenic proteins (BMP-1, -2, -3, and -6)
Reference(s):
15135, 14538, 14537
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
adherent, epithelial-like cells growing in monolayers; image; image
Medium:
85% McCoy's 5A + 15% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; cells secrete particulate matter consisting of protein
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 43 hours
Harvest:
cell harvest of about 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human highly rearranged hyperdiploid karyotype with 16% polyploidy - 56(52-58)<2n>X, -X, -2, -2, +3, -6, -8, +10, -12, +14, +18, -19, +20, -21, -21, -22, +15mar, add(1)(p22), add(3)(q27)x2, add(4)(p15), add(6)(p21), add(7)(p22), add(8)(q24), add(9)(q35), add(11)(p15), add(12)(p13), add(13)(q14), add(14)(p12)x2 - 13q14 breakpoint is associated with osteosarcomas - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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