CAPAN-1
ACC 244
Cell line:
CAPAN-1
DSMZ no.:
ACC 244
Species:
human (Homo sapiens)
Cell type:
pancreatic ductal adenocarcinoma
Origin:
established from the liver metastasis of a pancreatic ductal adenocarcinoma in a 40-year-old man in 1974; described as growing in nude mice forming metastases, as resistant to 5-FU, secreting cytokines and to express cystic fibrosis transmembrane conductance regulator (CFTR) and to carry mutations in KRAS, SMAD4 and TP53
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split 1:2 to 1:4 every 6-10 days using trypsin/EDTA; after thawing, viability may drop below 60% temporarily and initial growth could be slow
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50-100 hours
Harvest:
cell harvest of about 1.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
apparently human karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list