CAPAN-2
ACC 245
Cell line:
CAPAN-2
DSMZ no.:
ACC 245
Species:
human (Homo sapiens)
Cell type:
pancreatic ductal adenocarcinoma
Origin:
established from the head of the pancreas of a 56-year-old man with pancreas adenocarcinoma in 1975; described as growing in nude mice and to carry mutations in KRAS, CDKN2A and TP53
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split semi-confluent (2/3 to 3/4 confluency) culture up to 1:10 every 7 days using trypsin/EDTA (for 10-20 min); adherence may require several hours or overnight
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50-70 hours
Harvest:
cell harvest of about 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 6% polyploidy - 68-73<3n>XX, -Y, +1, +1, +4, +5, +7, +11, -12, -14, -17, -18, -22, +3mar - del(1)(p11p33)x2, add(11)(p11), der(11)t(?8;11)(q12;p11)x2, i(14q)x2, add(21)(p11)x2, 1-2DM(24%) - sideline with +1, i(1q)x2, del(1)(q11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list