DAN-G
ACC 249
Cell line:
DAN-G
DSMZ no.:
ACC 249
Species:
human (Homo sapiens)
Cell type:
pancreas carcinoma
Origin:
derived from nude mouse xenografts
initiated from a tumor cell line which was established in 1985 from a 68 year-old female patient with pancreas carcinoma
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 33 hours
Harvest:
cell harvest of about 1-3 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human irregular hypotriploid karyotype with 3.5% polyploidy - 59(52-61)<3n>der(X), -X/Y, -X/Y, -1, -2, -3, -4, -5, +6, +7, -9, -10, -13, -16, -17, -18, -20, -22, -22, +4mar, add(2)(p?16), add(4)(q25), i(5p), add(6)(p22), add(7)(p11/13), der(7)t(3;7)(q11;p11), del(7)(p13), add(8)(p11), add(9)(p21), add(12)(p11), i(13q), der(14)t(14;?22)(q32;q12), add(15)(q26), der(19)t(19;hsr)(p13;?), add(20)(q13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list