A-204
ACC 250
Cell line:
A-204
DSMZ no.:
ACC 250
Species:
human (Homo sapiens)
Cell type:
rhabdomyosarcoma
Origin:
established from the rhabdomyosarcoma of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice consistent with embryonic rhabdomyosarcoma
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% McCoy's 5A + 10-15% h.i. FBS
Subculture:
split confluent culture 1 x per week at up to 1:5 using trypsin/EDTA; seed out directly in a 80 cm2 culture flask, cells will reach confluency within 5-8 days; cells may clump after trypsinization and may be difficult to disperse
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 36 hours
Harvest:
cell harvest of about 5-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype with tetraploid sideline (31%) and 3.5% polyploidy - 46(41-46)<2n>XX - no consistent chromosome abnormality detected
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list