LAMA-87
ACC 270
Cell line:
LAMA-87
DSMZ no.:
ACC 270
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis (derivative of LAMA-84)
Origin:
the parental cell line LAMA-84 (DSM ACC 168) has been established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) in blast crisis; subclone LAMA-87 was obtained after subcutaneous transplantation of LAMA-84 cells into estrone-treated nude mice; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 every 2-3 days; spent medium should be exchanged every 24-48 hours even with splitting of the cells; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximal density at ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype - 60-64<3n>XX, -X, -2, -3, -7, +8, -10, -13, -14, -15, -16, -18, +19, -20, +22, der(9)t(9;22)(q34;q11)x2, del(12)(p12), del(12)(q14), add(19)(p13), add(?19)(p11), der(22)t(9;22)(q34;q11)x4 - four copies of Ph present in most cells - resembles published karyotype (distinguishable from LAMA-84 by 12p- marker)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list