A-2
ACC 276
Cell line:
A-2
DSMZ no.:
ACC 276
Species:
fish - Poeciliid (Xiphophorus xiphidium)
Cell type:
non-tumorous embryonal cells
Origin:
derived from non-tumorous epithelial-like embryonal cells of the sweetwater poeciliid fish (platyfish) Xiphophorus xiphidium; origin: Rio Purificacion; strain: YF1
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% mixture of Ham's F12 + DMEM (at 1:1) + 15-20% h.i. FBS
Subculture:
split culture 1:3 about 2-3 times per week before cells reach confluency; cells grow loosely adherent and can be detached from the plastic surface by shaking or pipetting; old cultures tend to form foci and lose adherence, but the cells remain attached to each other and are difficult to disperse
Incubation:
at 26-28 °C with 10% CO2
Doubling time:
about 2-3 days
Harvest:
maximal density of 6-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Xiphophorus xiphidium species.
Viruses:
ELISA: reverse transcriptase negative
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list