BHT-101
ACC 279
Cell line:
BHT-101
DSMZ no.:
ACC 279
Species:
human (Homo sapiens)
Cell type:
thyroid carcinoma
Origin:
established from the lymph node metastasis of a 63-year-old woman with anaplastic papillary thyroid carinoma; cells were described in the literature to not produce hormones, but to be partly positive for thyroglobulin- and thyroxine (T4)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS + 0.5% human serum (+ 0.005 IU/ml TSH + 5 µg/ml human insulin - cells grow also with these latter supplements)
Subculture:
seed out ca. 0.5-1 x 106 cells/80cm2; split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 2-3 days
Harvest:
cell harvest of 3-4 x 106 cells/80cm2
Storage:
frozen with 69.5% medium, 20% FBS, 0.5% human serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
apparently human hypertetraploid karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list