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DetailsCatalogue
Cell line:
JURKAT
DSMZ no.:
ACC 282
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features
Reference(s):
14944
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at about 1 x 106 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 25-35 hours
Harvest:
maximal density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, TCRalpha/beta +, TCRgamma/delta -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting
Cytogenetics:
human flat-moded hypotetraploid karyotype with 7.8% polyploidy - 87(78-91)<4n>XX, -Y, -Y, -5, -16, -17, -22, add(2)(p21)/del(2)(p23)x2 - sideline with additional der(5)t(5;10)(q11;p15), del(9)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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