U-138-MG
ACC 291
Cell line:
U-138-MG
DSMZ no.:
ACC 291
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established from the glioblastoma of a 47-year-old man
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
fibroblastoid fusiform cells growing as mono- or multilayers
Medium:
90% Ham's F10 + 10% h.i. FBS
Subculture:
seed out at about 1-2 x 106 cells/80cm2 flask; split confluent culture 1:5 to 1:10 every 4-8 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
cell harvest of ca. 4-6 x 106/80cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 20% polyploidy - 60(57-63)<3n>XYY, -3, -5, -6, -8, -9, -10, -11, -13, -14, -17, -19, +2mar, add(4)(p15), del(6)(q15), dup(7)(q35q21), add(11)(p15), add(12)(p11), add(14)(q32)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list