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DetailsCatalogue
Cell line:
HL-60
DSMZ no.:
ACC 3
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described in the literature to be responsive to DMSO, phorbol ester TPA and other reagents (e.g. expression of TNF alpha) and to carry amplified MYC gene; present cells are apparently tetraploid derivates of hypodiploid original where MYC was amplified in dmin (instead of hsr)
Reference(s):
14548, 14437, 14438, 14443, 15215
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round, single cells in suspension; image; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 1 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40 hours
Harvest:
maximal density of 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of MDH, NP
Cytogenetics:
human flat-moded hypotetraploid karyotype with hypodiploid sideline and 1.5% polyploidy - 82-88<4n>XX, -X, -X, -8, -8, -16, -17, -17, +18, +22, +2mar, ins(1;8)(p?31;q24hsr)x2, der(5)t(5;17)(q11;q11)x2, add(6)(q27)x2, der(9)del(9)(p13)t(9;14)(q?22;q?22)x2, der(14)t(9;14)(q?22;q?22)x2, der(16)t(16;17)(q22;q22)x1-2, add(18)(q21) - sideline with: -2, -5, -15, del(11)(q23.1q23.2) - c-myc amplicons present in der(1) and in both markers
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 - , HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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