Go to ContentTo Startpage
DetailsCatalogue
Cell line:
CAL-51
DSMZ no.:
ACC 302
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype
Reference(s):
14563
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris; image; image
Medium:
80-90% Dulbecco's MEM (4.5 g/L glucose) + 10-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split culture 1:2 to 1:4 once or twice a week using trypsin/EDTA before cells reach complete confluency
Incubation:
at 37 °C with 10% CO2
Doubling time:
50-60 hours
Harvest:
cell harvest of ca. 15-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting
Cytogenetics:
human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
Print data sheet