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DetailsCatalogue
Cell line:
293
DSMZ no.:
ACC 305
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS); cell line also known as HEK-293 (human embryonic kidney-293)
Reference(s):
14573
Biosafety level:
1, GMO-S1
Risk assessment:
The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced.
Permissions and restrictions:
A, D
 
DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid cells growing as monolayer; image; image
Medium:
90-95% Dulbecco's MEM (with 25mM HEPES) + 5-10% h.i. FBS
Subculture:
seed out at about 2-3 x 106 cells/25 cm2; split confluent culture 1:5 to 1:6 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask)
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 24-30 hours
Harvest:
cell harvest of about 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 (+), cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH
Cytogenetics:
human near-triploid karyotype with 6% polyploidy - 69(64-69)XX?X, +1, +6, -10, -13, -16, -18, -20, +21, +22, +mar, add(?X)(p22), inv(1)(p35q43), del(1)(p35), i(5p), del(6)(q11), add(8)(q24), add(13)(p13), add(17)(qter), der(?)t(5;?)(q13;?)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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