CPC-N
ACC 306
Cell line:
CPC-N
DSMZ no.:
ACC 306
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established from the pleural effusion metastasis of a patient with small cell lung carcinoma in 1987; listed in the CCLE (ref 18232) with genome and protein analyses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
seed out in a small culture flask, or, if possible to determine cell number: at 2 x 106 cells in 5-6 ml; split culture 1:2 every 3-4 days; the viability remains at max. 60%,
large amounts of cell debris may prevail in the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
maximal density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 16% polyploidy - 49(47-51)<2n>XY, +6, +15, +20, add(1)(p36), i(3q), del(6)(q1?3q2?5), der(8)t(8;17)(p23;q21), add(13)(p11)del(13)(q12q22), add(15)(p12), ins(16;8)(p13;HSR?q24) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list