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DetailsCatalogue
Cell line:
SW-480
DSMZ no.:
ACC 313
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the tumor of a 50-year-old Caucasian man with colon adenocarcinoma (grade 4, Duke class B)
Reference(s):
14713
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
endothelial-like adherent cells, mostly bipolar with microvilli growing in monolayers; image; image
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at about 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
25-30 hours
Harvest:
cell harvest of about 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, MDH
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 58(51-58)<3n>XX, -Y, -1, -3, -4, -5, -6, +7, -8, -10, -12, -15, -18, -19, +20, -22, der(1)t(1;9)(q22;q12), t(2;12)(q35;q13.1), del(5)(q13.2q32), add(7)(q35), dup(7)(q11.22q21.3), der(8)t(1;8)(p11;p11)t(1;9)(q22;q12), add(10)(p14), inv(14)(q13q32), del(18)(q12.2), der(19)t(6;19)(p11;q13), der(20)t(14;20)(q13;p13)x2 - identified by large submetacentric der(2)t(2;12) marker
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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