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DetailsCatalogue
Cell line:
HT-1080
DSMZ no.:
ACC 315
Species:
human (Homo sapiens)
Cell type:
fibrosarcoma
Origin:
established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972; patient did not receive any chemo- or radiotherapy
Reference(s):
14881
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like adherent cells growing as monolayers; image; image
Medium:
90% Dulbecco's MEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 in 7-10 ml medium; split confluent culture about 1:10 every 3 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 25 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, NP
Cytogenetics:
human near-diploid karyotype with 24% polyploidy - 46<2n>XY, der(5)t(5;5)(p15;q32), der(11)t(10;11)(q24;q11) - sideline with -13, del(1)(p32), del(1)(q24) - 5p15 is a putative recurrent breakpoint in fibrosarcoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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