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DetailsCatalogue
Cell line:
BFTC-905
DSMZ no.:
ACC 361
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
established from a 51-year-old Chinese woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan
Reference(s):
14430, 15028, 18168
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers, forming large islets; image; image
Medium:
80-90% Dulbecco's MEM + 10-20% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split culture before reaching confluency 1:3 to 1:5 once or twice a week using trypsin/EDTA (for 5-10 min)
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 60-70 hours
Harvest:
cell harvest of ca. 10-15 x 10 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS and 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline)
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH
Cytogenetics:
human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p13) - i(5p) and loss of 9p associated with transitional cell carcinoma - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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