MOLT-4
ACC 362
Cell line:
MOLT-4
DSMZ no.:
ACC 362
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-3 (DSMZ ACC 84). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.3 x 106 cells/ml; split ratio of ca. 1:2 to 1:3 every 2-3 days; viability may transiently decrease after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximal density at ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, cyCD3 +, smCD3 -, CD5 +, CD6 +, CD7 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype - 89-99<4n>XXYY, +4, +7, +8, +20, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2 - closely related to MOLT-3
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list