KYSE-150

ACC 375

Cell line:

KYSE-150

DSMZ no.:

ACC 375

Species:

human (Homo sapiens)

Cell type:

esophageal squamous cell carcinoma

Origin:

established from the poorly differentiated squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice; extensively characterized cell line (see cellosaurus), listed in CCLE (ref 18232)

Reference(s):

14646, 14954, 14955, 15006, 18232

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epitheloid cells growing as adherent monolayer; image; image

Medium:

49% RPMI 1640 + 49% Ham's F12 + 2% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/175 cm²; split confluent culture up to 1:20 every 3-4 days using trypsin/EDTA; cells grow very fast, a FBS amount around 2-5% is sufficient

Incubation:

at 37 °C with 5% CO₂

Doubling time:

below 25 hours (even less when serum amount is increased)

Harvest:

cell harvest of ca. 15 x 10⁶ cells in a 175cm² flask

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p11), der(11)add(11)(p1?)add(11)(q24), del(1)(p11), del(12)(q21), del(15)(q23q25), add(19)(q13)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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