Species:
human (Homo sapiens)
Cell type:
acute myeloid (eosinophilic) leukemia
Origin:
established in 1984 at diagnosis from the peripheral blood of a 33-year-old man with acute myeloid (eosinophilic) leukemia (AML) following hypereosinophilic syndrome; we found that EOL-1 carries a KMT2A (MLL) partial tandem duplication; described in the literature to carry the fusion FIP1L1-PDGFRA. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
(relatively small) round cells growing in suspension, singly or in small clusters;
image;
image;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; start culture with 20% FBS; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
maximum density of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 7.5% polyploidy - 50(48-51)<2n>,XY, +4, +6, +8, +19, del(4)(q12)x2, del(9)(q22) - del(4) effects cryptic FIP1L1-PDGFRA fusion - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
