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DetailsCatalogue
Cell line:
CMK
DSMZ no.:
ACC 392
Species:
human (Homo sapiens)
Cell type:
acute megakaryocytic leukemia
Origin:
established from the peripheral blood of a 10-month-old boy with Down's syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985
Reference(s):
14685, 14921
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
single, multiformed, large cells growing in suspension, sometimes loosely adherent (<3%); 2-3% giant, multinucleated cells; image; image; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days; viability may be consistently between 60% and 80% and culture may always contain a certain amount of cell debris; only exchange spent medium partially during the first week; cultivation in 12- or 24-well plates could be of advantage
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximal density at ca. 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, NP
Cytogenetics:
human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21), add(8)(q13-21), del(8)(q11), del(9)(p21)x2, add(9)(q11)x2, del(10)(q22q24), der(11;17)(q10;q10), der(11)dup(11)(p13p15)t(5;11)(q11;p15)x1-2, del(11)(q23), add(12)(p13)x2, add(17)(p1?), add(18)(q23)x2-3, add(19)(p13), der(20)t(1;20)(q2?5;q1?2)x2, add(22)(q13) - disomic rearrangement of 20q1 close to common deleted segment, together with additional rearrangements of ch 20 present in markers - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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