BE-13 (subclone of PEER)
ACC 396
Cell line:
BE-13 (subclone of PEER)
DSMZ no.:
ACC 396
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
tetraploid subclone of cell line PEER (DSMZ ACC 6); see there
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at about 1 x 106 cells/ml in a 24- or 6-well plate; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 2-4 days; (cells grow clearly better in 24- or 6-well plates than in flasks)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
maximal density at ca. 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to PEER
Cytogenetics:
human flat-moded hypotetraploid karyotype - 81-89<4n>XXXX, +1, +4, -9, -9, -10, -16, -21, del(1)(q13.2), add(1)(p11-21), del(4)(q11q31.2)/i(4p)x2, i(4q)del(4q31.2)/i(4q)add(4)(q31)x2, del(5)(q14q21)x2, del(6)(q22)x2, del(9)(p22)x2, der(17)t(17;?21)(p11;q11)x2 - 5q- and apparent 9p22-pter nullisomy
Molec. Genetics:
expression of fusion gene NUP214-ABL1 confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list