HT-1376

ACC 397

Cell line:

HT-1376

DSMZ no.:

ACC 397

Species:

human (Homo sapiens)

Cell type:

urinary bladder carcinoma

Origin:

established from a transurethral resection of invasive, moderately pleomorphic (grade 3) bladder transitional cell carcinoma of a 58-year-old woman who had not received chemo- or radiotherapy

Reference(s):

14579, 14882

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epitheloid adherent cells growing in monolayers; image; image

Medium:

85% DMEM (4.5 g/L glucose) + 15% h.i. FBS + 2 mM L-glutamine

Subculture:

split confluent cultures 1:5 once a week using trypsin/EDTA; seed out at ca. 1.5 x 10⁶ cells/80 cm²; maximal density at ca. 60 x 10⁶ cells/175 cm²

Incubation:

at 37 °C with 10% CO₂

Doubling time:

ca. 60 hours

Harvest:

cell harvest of ca. 40 x 10⁶ cells/175 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded pentaploid karyotype with 8% polyploidy - 111(102-116)<5n>XX/XXX, +3, +3, +5, +5, -6, +7, +8, +8, -12, -13, -13, -14, -14, -15, -15, -15, -15, -16, -17,-17, -19, -19, +20, -22, -22, -22, +6-8mar - del(2)(q25), add(3)(q27)x2, del(3)(p11p14), i(5q), add(5)(p15), del(5)(p13), add(5)(q1?3;hsr), del(6)(q25), add(7)(p22), del(7)(q11), add(8)(p23)x2, i(9q)x2, del(9)(p21), del(10)(q24), add(11)(p15), add(12)(p13)x1-2, del(12)(p11), der(13)t(13;?)(p11;?)t(?;1)(?;q11), add(17)(p1?), add(18)(p11) - sideline with i(5p) - 3p, 5p/q10, +7, 8p, 9p, 10q, 11p, 17p rearrangements associated with bladder TCC - matches published karyotypes

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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