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DetailsCatalogue
Cell line:
BHY
DSMZ no.:
ACC 404
Species:
human (Homo sapiens)
Cell type:
oral squamous cell carcinoma
Origin:
established from the tumor of a 52-year-old Japanese man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice
Reference(s):
14652
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers; image; image
Medium:
90% Dulbecco's MEM (4.5 g/L gluocse) + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 in 15 ml medium; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 70 hours
Harvest:
cell harvest of ca. 7 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of AST, NP and recently by STR fingerprinting
Cytogenetics:
human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del(5)(q13), add(5)(p15)x1-2, der(8;10)(q10;q10), add(9)(q34), add(9)(p11), del(10)(p13), del(10)(q23), der(11)t(1;11)(q11;q25), add(12)(p11), add(12)(p11), add(12)(q13-21), add(13)(p11), add(16)(q24), del(16)(p13), add(17)(p12), add(18)(p11), add(19)(q13) - 3-10 dmin counted in some cells
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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