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DetailsCatalogue
Cell line:
42-MG-BA
DSMZ no.:
ACC 431
Species:
human (Homo sapiens)
Cell type:
glioma
Origin:
established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)
Reference(s):
14846
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes; image; image
Medium:
80% mixture of RPMI 1640 + MEM (with Earle's salts) (at 1:1) + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; saturation density at ca. 0.6 x 105/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of maximal 0.3-0.5 x 105/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of LDH, MDH, NP
Cytogenetics:
human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)add(2)(p2?4), del(3)(q13.3), add(4)(p11), add(5)(p1?5), add(6)(q2?)x2, add(7)(q11)x2, add(7)(q3?), del(9)(p21p22)x2, del(11)(p11)x2, inv(12)(q12q21)x2, der(12)del(p11)t(7;12)(q21;q1?4)x2, add(13)(p11)x2, add(14)(q32)x2, add(21)(p11)x2
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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