22RV1
ACC 438
Cell line:
22RV1
DSMZ no.:
ACC 438
Species:
human (Homo sapiens)
Cell type:
prostate carcinoma
Origin:
cell line was derived from a human prostate carcinoma xenograft (CWR22R) that was serially propagated in nude mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft; the cells produce xenotropic murine leukemia virus-related virus (XMRV) (see risk assessment)
Biosafety level:
1
Risk assessment:
The cell line was shown to carry more than 10 copies of the xenotropic murine leukemia virus related virus integrated as proviruses into the eukaryotic genome. Active viruses are secreted into the cell culture supernatant. Murine retroviruses can cause leukemia, lymphoma or other tumors.
A relation to analogous diseases in human could not be shown. Thus, the viruses and the cell line was categorized bio safety level 1. However, the German Central Committee on Biological Safety (ZKBS) classified this cell line risk group 2 when the cells are used for genetic engineering.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
40% RPMI 1640 + 40% DMEM + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells in a 175 cm2 flask; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA for ca. 10 min
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175cm 2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy - 50(48-52)<2n>XY, +1, +7, +8, +12, i(1q), der(2)t(2;4)(p1?4;q3?3)del(2)(q11q3?1), der(4)t(2;4)(p1?4;q3?3), add(6)(q15), add(14)(q24) - sideline with del(1)(q21), +3, +18 - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV -, XMRV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list