CAL-72
ACC 439
Cell line:
CAL-72
DSMZ no.:
ACC 439
Species:
human (Homo sapiens)
Cell type:
osteosarcoma
Origin:
established from a chemotherapy-resistant tumor sample obtained from the left knee of a 10-year-old boy with anaplastic osteoblastic sarcoma (Rosen grade IIB) in 1989
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
fibroblastoid cells growing adherently in monolayers; image
Medium:
90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite
Subculture:
seed out at ca. 1 x 106 cells/80 cm2;
split confluent culture 1:3 to 1:10 every 4-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5-10% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 7 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human, bimodal hyperdiploid/hypertetraploid karyotype with 12% polyploidy - 45-56<2n>X, -Y, +1, +1, -2, -2, +8, +11, -13, -15, +19, -20, -20, -22, -22, +15-20mar, +dmin, add(X)(q21), der(1;19)(q10;q10), add(1)(p3?5)add(1)(q3?), add(3)(q2?9)x2, add(6)(q11), del(7)(q11), add(8)(q24), add(8)(q11), add(11)(p1?), del(11)(q13), add(14)(q3?)x2, add(16)(p11), add(16)(q23), add(19)(p11), add(21)(p11) (hyperdiploid clone) - additional rearrangements, including der(1;8)(q10;q10) and idic(8;?)(p10;?) present in hypertetraploid clone
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list