CAL-27
ACC 446
Cell line:
CAL-27
DSMZ no.:
ACC 446
Species:
human (Homo sapiens)
Cell type:
tongue squamous cell carcinoma
Origin:
established from the poorly differentiated squamous cell carcinoma of the tongue removed from a 56-year-old man before treatment in 1982
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out 1-2 x 106 cells in a small (25cm2) culture flask; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; maximum density of ca. 14-18 x 106 cells/80cm2 flask
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 20-40 hours
Harvest:
cell harvest of ca. 8-12 x 106 cells/80cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 3% polyploidy - 41(38-45)<2n>X, -Y, -4, +7, -10, -11, -18, -20, -21, -22, +2-3mar, add(3)(q21), i(3q), i(7p), del(8)(p21), i(9q), add(13)(p11), add(14)(p11) - sideline with additional der(3) and der(7) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list