KE-37
ACC 46
Cell line:
KE-37
DSMZ no.:
ACC 46
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-92.5% RPMI 1640 + 7.5-10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/ml; after thawing, cells should be cultured with 20% FBS for the first 1-2 weeks; maintain at about 1-2 x 106 cells/ml and split saturated culture 1:2 to 1:3 every second day
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 2-3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD2 +, cyCD3 +, smCD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype - 91(86-92)<4n>XXYY, +8, -14, -14, t(7;12)(q32-33;p12-13)x2, der(8)t(8;14)(q24;q11)x4, der(14)t(8;14)(q24;q11)x2 - resembles published karyotype - carries 4 copies of der(8)t(8;14) associated with T-ALL - normal ch 14 present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list