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DetailsCatalogue
Cell line:
PC-3
DSMZ no.:
ACC 465
Species:
human (Homo sapiens)
Cell type:
prostate carcinoma
Origin:
established from the bone marrow metastasis isolated post-mortem from a 62-year-old Caucasian man with grade IV prostate cancer (poorly differentiated adenocarcinoma) after androgen suppression therapy; described in the literature to form tumors in nude mice, to grow in soft agar, and to be unresponsive to androgen treatment
Reference(s):
14644, 14832, 15188
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing adherently in monolayers or in multilayer foci
Medium:
45% Ham's F12 + 45% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of 3-5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human highly-rearranged flat-moded hypotetraploid karyotype with 3% polyploidy - 80-90<4n>XX/XXX, -Y, -1, -1, -2, -2, +3, +3, -4, -5, +7, -8, -8, -10, -10, -10, -10, -12, -13, +14, -15, -15, -16, -16, -17, -18, -18, -22, +18-21mar, +1-2dm, del(1)(p34p35), add(1p?32), add(3)(p1?), add(3)(q13), add(3)(q21)x2, del(5)(p1?)x1-2, add(9)(p1?1), del(9)(p21)x1-2, add(12)(q24), add(13)(p11), del(17)(p11), add(19)(p13) - sideline with der(3)add(3)(p2?5)add(3q2?), del(4)(q2?4q2?8), add(7)(q11), add(8)(p1?), add(9)(q34) - extensive genomic amplification of c-MYC in hsr present in markers - distantly related to published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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