SIG-M5
ACC 468
Cell line:
SIG-M5
DSMZ no.:
ACC 468
Species:
human (Homo sapiens)
Cell type:
acute monocytic leukemia
Origin:
established in 1995 from the bone marrow of a 63-year-old man with acute myeloid leukemia of monocytic origin (AML FAB M5a) at diagnosis
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% Iscove's MDM + 20% h.i. FBS
Subculture:
cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; after thawing, it is advisable to initially grow the cells in 12- or 24-well plates; cell viability remains continuously at or below 70%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 72 hours
Harvest:
maximum density of about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3-, CD4-, CD13-, CD14(+), CD15+, CD19-, CD33+, CD34-, HLA-DR-, cyCD68-, cyCD3-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid/hypotetraploid karyotype with 4% polyploidy - 78(77-81)<3n>XX/XXY, +1, +2, +3, +4, +5, +8, -9, -10, +12, +15, +16, +17, +18, +21, +22, +0-2mar - i(1q), add(17)(p1?1) - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list