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DetailsCatalogue
Cell line:
HCC-33
DSMZ no.:
ACC 487
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established from the pleural effusion of a 52-year-old man with small cell lung carcinoma; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-33BL)
Reference(s):
14567, 15046
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round to ovoid cells growing singly or in clumps
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:3 every 2-5 days; seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; after thawing, cell clumps should be carefully resuspended to encourage growth
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin + (dot-like expression, no regular filaments), desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded hypotriploid karyotype with 8% polyploidy - 61-68<3n>XX, -Y, +1, +1, +3, -10, -12, -13, +17, -20, -22, +3-5 mar, ider(1)(q10)del(1)(?q12?q43)x2, add(1)(p36), del(1)(p32)x1-2, dup(2)(p23p24), der(3;17)(q10;q10)x1-2, add(8)(p11), del(9)(q21), der(12)t(11;12)(q13.4;q24.3), add(13)p11) - sideline with add(5)(p11), add(15)(p11), add(17)(p1?), del(22)(q12) - variable dmin present in most cells - dup(2) includes MYCN locus
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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