Species:
human (Homo sapiens)
Cell type:
natural killer lymphoma
Origin:
established from the peripheral blood of a 50-year-old man with non-Hodgkin lymphoma (large granular lymphocytic) in 1992; cells were described as having azurophilic granula and strong cytotoxic NK activity. Used in clinical trails against cancer. Variants NK-92ci and NK-92mi are not available at DSMZ. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
For-profit organizations require a license from the owner of the cell line prior of its use. The material or its products must not be transferred to third parties for any reason. Any proposed commercial use of these cells must first be negotiated with:
ImmunityBio, Inc.
Medium:
75% alpha-MEM (with ribo- and deoxyribonucleosides) + 12.5% h.i. FBS + 12.5 % h.i. horse serum + 2 mM L-glutamine + 5 ng/ml IL-2
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.6 x 106 cells/ml; split saturated culture 1:2 every 2 days; after thawing, it is recommended to first culture the cells in 24-well-plates; viability may not surpass 60-70%; cells grow mainly in large clusters, outside these clusters a significant amount of cell debris and dead cells are always visible
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximum density of about 0.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 -, CD4 -, CD5 -, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 4% polyploidy - 80(75-82)<4n>XXYY, +3, -4, -8, -9, -10, +12, +12, -14, -17, -17, -22, der(3;5)(q10;p10), i(7q), der(7)t(7;7)(p21;q11), der(8)t(8;8)(p23;q13), der(8)t(8;15)(p23.2;q14)x1-2, del(9)(p12p21), der(9)t(9;13)(p12;q11), der(10)t(10;18)(p12;q11)t(10;11)(q24;p13), der(12)t(11;12)(q13.4;q11)x1-2, der(13;19)(q10;q10), del(15)(q14)x1-2, del(16)(q23), der(16)t(11;16)(q22.2;q22), del(20)(?q12q13.1), der(Y)t(Y;17)(p11.3;q11), der(Y)t(Y;17)(p11.3;q11)t(11;17)(p1?2;q25) - sideline with der(9;21)(p10;q10)
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
