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DetailsCatalogue
Cell line:
HCC-15
DSMZ no.:
ACC 496
Species:
human (Homo sapiens)
Cell type:
non-small cell lung carcinoma
Origin:
established from the tumor of a 47-year-old man with non-small cell lung carcinoma (subtype squamous carcinoma); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-15BL)
Reference(s):
14567, 15046
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
adherent epitheloid cells growing as monolayer; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:4 to 1:6 every 4-5 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human hypodiploid karyotype with 16% polyploidy - 37(34-38)<2n>X, -X, -4, -13, -14, -17, -18, -19, -20, -22, +1-2mar, del(3)(q13), der(5;19)(q10;q10)add(19)(q13), add(9)(p2?), del(10)(p12-13), add(12)(q24.3), del(20)(?p12), add(22)(q1?) - sideline with add(8)(p12), del(7)(q21)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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