Leibniz Institute DSMZ: Details

Cell line:

OPM-2

DSMZ no.:

ACC 50

Species:

human (Homo sapiens)

Cell type:

multiple myeloma

Origin:

established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG lambda) in leukemic phase (relapse, terminal) in 1982; described in the literature to carry t(4;14) leading to IGH-FGFR3 (IGH-MMSET) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Reference(s):

14650, 14486, 18147, 18187

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

single, round to polygonal cells in suspension; image; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/ml in a 12- or 24-well-plate; maintain at 0.3-0.7 x 10⁶ cells/ml; split 1:2 to 1:4 every 2-3 days; viability may decrease after thawing, keep at 20% FBS for the first week

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 50-60 hours

Harvest:

maximal density at 1.0 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, PCR assays

Immunology:

CD3 -, CD10 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypertriploid/hypotetraploid karyotype - 78(77-82)<3n>XX-X, +1, +1, +1, +2, +4, +5, -7, +9, +11, -14, +15, +16, +18, +19, -21, +2-3mar, add(1)(p11), add(1)(q11)x2, del(1)(p11)x2, del(1)(q32), t(4;14)(p16;q32.3), der(4)t(4;14)(p16;q32.3), add(5)(q11), add(5)(q15-21), add(5)(q35), der(8)t(1;8)(?q32;q24)x1-2, add(14)(q32.1), der(22)t(1;22)(q11;p13)x2 - sideline with del(7)(p15), del(10)(q22) - resembles published karyotype - carries cryptic t(4;14) associated with fusion of IGH with FGFR3 (MMSET)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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