H-1184
ACC 502
Cell line:
H-1184
DSMZ no.:
ACC 502
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established in 1985 from the lymph node from a 42-year-old white man with metastatic small cell lung carcinoma prior to treatment; corresponds to NCI-H1184; matched B-lymphoblastoid cell line is available (NCI-BL1184, DSMZ ACC 896)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
small epitheloid cells growing adherently in monolayers or in suspension as clusters
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/cm2 in a 80 cm2 flask; split saturated culture 1:2 to 1:4 every 5-8 days using trypsin/EDTA; after recovery change medium sparingly
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximum density of ca. 20-25 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy - 57(53-59)<2n>XY/XXY, +1, +3, +5, +8, +9, -10, -10, +11, +12, -14, +16, +20, +22, +6mar, add(1)(p34), add(1)(p11), add(3)(p14), add(3)(q21-22), add(5)(p11), add(5)(q11), add(8)(q21), add(11)(q25), add(12)(q24)x1-2, der(12)t(11;12)(q11;q24), add(13)(p11), del(?13)(q12q21), del(16)(p12p13), der(?22)t(14;22)(q21;q13)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list