CAL-29
ACC 515
Cell line:
CAL-29
DSMZ no.:
ACC 515
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
established from the primary lesion of a fatally invasive, metastatic transitional cell carcinoma of the bladder (grade IV, stage T2) from an 80-year-old woman before treatment
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelioid cells, some with spindle-like extensions growing adherently in monolayers
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximal density of ca. 6.0 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 8% polyploidy - 71(63-74)<3n>XX/XXX, -4, +9, -11, -13, -17, +20, +3mar, +1-6 dmin, i(3q), add(7)(q22)x1-2, del(7)(q32)x2, del(9)(p21), del(10)(p13), add(12)(p1?3), add(19)(p13)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list