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DetailsCatalogue
Cell line:
KCL-22
DSMZ no.:
ACC 519
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described in the literature to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation
Reference(s):
14690, 14691, 14484
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
round, single cells in suspension, sometimes in small clusters; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; split saturated culture 1:5 to 1:10 every 2-3 days; maximal density of ca. 2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of about 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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