KASUMI-2
ACC 526
Cell line:
KASUMI-2
DSMZ no.:
ACC 526
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990 at diagnosis
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1.5 x 106 cells/ml; start culture in 24-well-plates in the presence of 20% FBS (for the first 1-2 weeks); split saturated culture 1:2 to 1:6 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density of ca. 7 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 7 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD10+, CD13+, CD19+, CD20+, CD34(+), CD37-, CD38+, CD80-, CD138-, HLA-DR+, sIgG-, cIgG-, sIgM-, cIgM+, sIgkappa-, cIgkappa-, sIglambda-, cIglambda-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 3% polyploidy - 45(44-46)<2n>X, -Y, del(2)(p23.2/p21p23.1), t(3;14)(q27-28;q24), del(6)(q21), i(7q), del(13)(q1?3q1?4), der(19)t(1;19)(q23;p13) - sideline with idem 46, XY - carries der(19) partner of t(1;19) with TCF3 (E2A) rearrangement confirmed by FISH
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list