JURL-MK2
ACC 533
Cell line:
JURL-MK2
DSMZ no.:
ACC 533
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the peripheral blood of a 73-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1993; cells carry the Philadelphia chromosome; JURL-MK1 and JURL-MK2 are sister cell lines derived from the same patient
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round single cells growing in suspension, heterogenous in size, occasional giant cells
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3-4 days; reduced viability immediately after thawing (ca. 60-70%), but cells will recover thereafter
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 72 hours
Harvest:
cell harvest of ca. 2-3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 -, CD19 -, CD30 +, CD33 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 3.5% polyploidy - 81(77-82)<4n>XXYY, -2, -7, -12, +13, -14, -14, -16, -18, -19, -22, +1-3mar, add(9)(q1)x2, der(9)del(9)(p21.2)t(9;22)(q34;q11)x2, der(11)t(11;9;22)(p15;q34;q11)x2, add(17)(p13)x1-2, der(22)t(9;22)(q34;q11)x2 - carries two copies of Ph effecting BCR-ABL1 fusion together with additional FISH signals on 11p - does not match published karyotype but iteratively resembles JURL-MK1 (sister cell line)
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list