Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Morphology:
single round rather small cells growing in suspension;
image;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml in a 12-well-plate with 20% FBS; maintain at ca. 0.5-1.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 -, HLA-DR +, cyIgG -, cyIgM +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 4% polyploidy - 45(42-45)<2n>XX, -14, t(8;13)(q24;q21.2), t(11;19)(q23;p13), der(13)t(13;14)(p11;q11) - carries t(11;19) effecting KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion - matches published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
