NCI-H82
ACC 556
Cell line:
NCI-H82
DSMZ no.:
ACC 556
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established from the pleural effusion of a 41-year-old man with small cell cancer of the lung in 1978; listed in the CCLE (ref 18232) with extensive genome and protein analyses
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca 0.1-0.8 x 106 cells/ml; cell numbers can only be estimated due to the formation of large aggregates; we recommend not to disperse the aggregates too strong
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list