Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peritoneal effusion of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse, mixed small and large cell type; described to carry EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml in a 24-well-plate; maintain at ca. 0.4-1.0 x 106 cells/ml, best in 12- or 24-well-plates; split saturated culture 1:2 to 1:3 every 3 days; a certain amount of cell debris is always visible; a temporary reduction in cell viability may occur after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
cell harvest of ca. 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 2% polyploidy - 47(42-48)<2n>X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8;9)(q24;p13), der(9)t(8;19;9)(q24;q13;p13), dup(11)(q24q25), t(14;18)(q32;q21), der(22)t(?7;22)(?q32;p11) - sideline with dic(8;9)(q24;p13), ider(8)(q10)t(8;9(q24;p13) - matches published karyotype - carries t(14;18) effecting IGH-BCL2 fusion
Molec. Genetics:
expression of fusion gene PVT1::ZCCHC7 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
