HTC-C3
ACC 580
Cell line:
HTC-C3
DSMZ no.:
ACC 580
Species:
human (Homo sapiens)
Cell type:
thyroid carcinoma
Origin:
established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing as monolayer
Medium:
80% DMEM (4.5 g/L glucose) + 20% h.i. FBS
Subculture:
seed out 0.2-0.5 x 106 cells in 80 cm2; split confluent culture 1:2 once a week using trypsin-like enzyme (e.g. Tryple Express) (standard trypsin/EDTA is not sufficiently effective); note that viability may decrease rapidly after reaching confluency
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100 hours
Harvest:
cell harvest of ca. 12 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list