NIH-3T3
ACC 59
Cell line:
NIH-3T3
DSMZ no.:
ACC 59
Species:
murine (mouse, Swiss mouse) (Mus musculus)
Cell type:
embryo fibroblasts
Origin:
NIH Swiss mouse embryo; used in DNA transfection assays; described as contact inhibited
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS (or NCS)
Subculture:
seed out at 1-2 x 106 cells/175 cm2 flask;
do not allow culture to become completely confluent; split culture up to 1:10 every 4 to 6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 20 hours
Harvest:
cell harvest of about 20 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list